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KMID : 0861020230380050061
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Korea Journal of Herbology
2023 Volume.38 No. 5 p.61 ~ p.67
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Protective effect of Mori Fructus in HaCaT human keratinocytes exposed to UVB
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Lee Jong-Rok
Park Sook-Jahr
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Abstract
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Objective : Mori Fructus has been used to relieve thirst, and treat dizziness, tinnitus, and insomnia caused by poor
constitution. This study was performed to investigate the protective effect of the ethanol extract of Mori Fructus (MF)
in ultraviolet B (UVB)-induced apoptotic cell death in human keratinocyte cells.
Methods : MF was prepared by extracting 100 g of Mori Fructus in 1 L of 100% ethanol for 48 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure cell viability. Apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, and the expression of apoptosis-related proteins was observed by Western blot. Tyrosinase activity was measured with a colorimetric commercial kit
Results : MF promoted cell vitality and inhibited apoptosis of UVB-induced HaCaT cells. MF pretreatment reduced
TUNEL-positive cells and increased the expression of caspase-3 and ?9. MF also displayed antioxidant effect with
high radical scavenging ability. At 2 ㎎/㎖ concentration, the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid
(ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition rates were 55.3 ± 4.6% and 48.5 ± 1.3%, respectively. Furthermore, MF showed a concentration-dependent inhibitory effect on tyrosinase.
Conclusion : These results suggest that MF functions as a protective regulator in UVB-induced HaCaT cells by regulating apoptosis and partially exerting antioxidant effects. In addition, the tyrosinase inhibitory effect of MF shows the potential for MF to be used for skin pigmentation.
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KEYWORD
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Mori Fructus, UVB, apoptosis, caspase, tyrosinase
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